+32 2 265 09 20 lieven@gentaur.com
Follow us

reporter assay

multi-well plate compatible model 
appeared in Kronos series which measures changes in gene expression over time with bioluminescence of luciferase while culturing cells.


ATTO High throughput real-time luciferase assay system
WSL-1565 ATTO Kronos HT



High throughput real-time luciferase assay system Kronos HT
High throuput assay system for real-time gene expression 
monitoring of living cells


WSL-1565 Kronos HT is the high throughput support model of the assay 
system that measure the gene expression of living cells in real-time with the bioluminescence of  luciferase. Kronos Dio, which is a precedent model compatible with 35 mm dish, has been used  as a real-time luminescence measurement system with cell culturing function, but this time a new  lineup of model compatible with multiwell plate measurement is added. 


With this new product Kronos HT, we succeeded the know-how cultivated in the precedent model 
in order to measure the luminescence while culturing the cells, and further strengthened functions 
and performance for high throughput measurement. Please consider adopting Kronos HT for 
high-throughput measurement in biological clock analysis, change in drug response with time, 
cytotoxicity test etc.


NEW
• Cell culture function with temperature contol, humidifying 
and controlling CO2 concentration
• 24 and 96 well plate format
• Scan 2 plates with 2 detectors (PMTs)
• It does not shake or vibrate cells by scanning with 
detector moving and cuture plates not moving.


Biological clock, organ clock and cell clock... chronomedicine, research progresses in the world.


Reporter assay in real time with culturing cells


Reporter assay is used to measure the transcriptional activity of gene transcription 
regulatory sequences such as promoters, for researches on drug response and gene 
expression control, evaluation of gene transfer effi ciency, and so on. Among them, 
the method of measuring the amount of bioluminescence using bioluminescent 
enzyme luciferase using a device called a luminometer is superior in terms of high 
sensitivity and low background and is widely used.


Usually, transcription activity at a certain point (end point) is measured, but the 
assay is also becoming used for tracking transcription activity over time such as 
stimulation response, time course of transgene expression level, periodic variation 
of biological clock gene. Kronos HT is a system to optimize for such assays with the 
ability to monitor the bioluminescence over time as cells are cultured.


Time course of drug response
Long-term kinetics of activity inhibition
Monitoring of biological clock


• High sensitive and low noise detection by cooling of 
detectors (PMTs)
• Compatible with multicolor luciferase assay
• Easy setting with software on Windows PC, and 
displaying and saving measurement data in real time.
Biological clock, organ clock and cell clock... chronomedicine, research progresses in the world.

EzFluoroStain DNA


Light detecting with culturing cells
Sample stage (CO2
incubator)
Filter wheel
Cooled PMT
Multicolor assay
It can be applied to multicolor 
luciferase assay with fi lter automatic 
switching mechanism and ATTO's 
original color separation system 
(patent).
Maintain cell culture environment
The sample stage is a CO2
incubator with 
constant temperature, humidifi cation and 
CO2
control.


24/96 well plate compatible with adapter exchange
Although it is compatible with 24 well plates in the standard model, 
it is also able to use 96 well plates by simply replacing it with an 
adapter of an optional item.
High sensitive detection
Weak light can be detected by cooling 
the detector (photomultiplier tube, PMT) 
to 10°C to suppress thermal noise.
Effi cient measurement of multiple samples
Since two plates are scanned simultaneously by two 
detectors, more samples can be measured at shorter 
intervals.


Cell-friendly scanning mechanism
Since it has a mechanism to move the detectors 
without moving the sample stage, the cultured 
cells are not shaken or vibrated.
Heating and
humidifying the gas
Controlling
CO2
gas concentration
CO2
gas
Exchanged to a 96 well plate adaptor (option)
Sample stage (setting two of 24 well plates)
Software
Setting measurement conditions
You can set it by simple operation. Setting 
operation can be omitted by using the function 
to save and load the setting condition as a fi le.
Display measurement data in real time
Since the data under measurement is displayed 
in real time, you can check how the measured 
value changes over time. Measurement data 
is automatically saved and the data fi le can be 
taken out even during measurement.
Data processing
It has a smoothing function to remove 
noise component from data and smooth 
it, a multicolor calculation function to 
calculate each color emission value 
from measurement value of each fi lter, 
and a detrending function to extract 
rhythm variation of biological clock.


Data export


Since data can be exported as a CSV format 
fi le, it can be managed and edited with 
spreadsheet software such as Microsoft 
Excel or other analysis software. Also, you 
can export the graph list of each data as an 
image fi le.
Multicolor calculation
Smoothing
Detrending 
Export as a CSV fi le
Export as
a graph image fi le
Graph list display of all samples
Detailed graph list display
Main window
Setting window
Circadian rhythm of clock gene expression
Application
NF-κB response elemnt - TK promoter - SLG (green color emited luciferase) and TK promoter - 
SLR (red color emitted luciferase) expressed fi broblast cell line in 96 well clear bottom plate


Measurement time: 5s x 2 color / well, Interval time: 30min
(A) Transcriptional activity of NF-κB response element for 48h
(B) Transcriptional activity of TK promoter for 48h
(C) Normalized by activity of control (TK promoter)
(D) Ratio to NF-κB activity without TNF-α
Transcriptional activity of clock gene, mPer2
mPer2 promoter - Eluc (PEST) expressed fi broblast 
stable cell line in 24 well clear bottom plate
Measurement time: 5s / well, Interval time: 10min
Dual-color real-time luciferase assay of TNF-α induced NF-κB transcriptional activity
(A) (B)
(C) (D)


Drug response of transcription factor
Specifications


Model WSL-1565 Kronos HT
Detector Photomultiplier tube (10°C cooling) x 2 units, X-Y actuating
Measurement Photon counting method
Measurement time Integral time: 1 - 60 s/well, Measurement term: 1 h - 30 days
Plate format Clear bottom 24 well plate x 2
Clear bottom 96 well plate x 2 (Option), 35mm dish x 12 (Option)
Throughput
48 samples (24 well plate x 2)
192 samples (96 well plate x 2) with an optional item, 96well plate adaptor
12 samples (35mm dish x 12) with an optional item, 35mm dish adaptor
Incubation 
temperature control Room temperature + 5°C to 45°C (0.1°C step) at room temperature 20 - 28°C
CO2 gas control Control to 5% by CO2 gas mixer
Humidifi cation Setting of water reservoir in incubator and humidifi ed 5% CO2 gas fl owing 
intermittently by humidifying unit, Maintaining >90%RH
Multi-color assay Measurement with automatically selected optical fi lter to 2 colors
(There is also a model for 3 color separation. Request inquiries.)
PC requirements OS: Windows 10 / 8.1 / 7, 64 / 32 bit, Memory: more than 4 GB, HDD: more than 
20 GB free space, Interface: USB2.0 x 1 port
Dimensions, Weight
Main unit: 650 (W) x 520 (D) x 340 (H) mm, 40 kg
CO2 gas mixer: 160(W) x 300 (D) x 200 (H) mm, 4.8 kg
Humidifying unit: 170 (W) x 140 (D) x 115 (H) mm, 1.6kg
Power
Main unit: AC 100 - 240 V, 50 / 60 Hz, 400 W (max)
CO2 gas mixer: AC 100 - 240 V, 50 / 60 Hz, 100 W (max)
Humidifying unit: AC 100 - 240 V, 50 / 60 Hz, 24 W (max)
Code No. Model
3510140
WSL-1565 Kronos HT
Kronos HT main unit, Control software (for Windows PC), CO2
gas mixer, 
Humidifying unit, 24 well plate adaptor
Code No. Model
3510147 96 well plate adaptor
3510148 35mm dish adaptor
Option
CO2 Main unit PC Humidifying unit gas mixer
(PC is not included in standard components)
Real-time luciferase assay system
35 mm dish format model
AB-2550ATTO Kronos Dio




Code No. Model
3510120 AB-2550 Kronos Dio


> Reporter assay in real time with culturing cells
> Cell culture function with temperature control, humidifying and 
controlling CO2
gas concentration
> 35 mm dish format, 8 dishes
> Compatible with multicolor luciferase assay
> Easy setting with software on Windows PC, and displaying and saving 
measurement data in real time.
Model AB-2550 Kronos Dio
Detector PMT (Photomultiplier tube)
Measurement Photon counting method
Throughput 8 samples (35 mm culture dish x 8)
Measurement 
time 1 s - 60 min / dish
Incubator Temperature setting: 20°C (RT - 5°C) to 45°C (1°C step), CO2 concentration: 5% 
(fi x), Humidifi cation: Humidifi cation sponge installation
Multi-color 
assay Measurement with automatically selected optical fi lter to 3 colors
PC 
requirements
OS: Windows 10 / 8.1 / 7, 64 / 32 bit, Memory: more than 4 GB, HDD: more than 
20 GB free space, Interface: USB2.0 x 1 port
Dimensions, 
Weight 280 (W) x 400 (D) x 330 (H) mm, 16 kg
Power AC 100 - 230 V, 50 / 60Hz, 150W (max)
*PC is not included in standard components.e...